
Quick and easy ligation-free cloning
Fast, easy, and efficient, SBI’s Cold Fusion Cloning is an excellent choice for any cloning project. Whether you"re assembling multiple fragments of DNA or simply adding an insert or gBlock® to a vector, the Cold Fusion Cloning Kit will take you to transformation-ready DNA in a single step—just incubate your DNA fragment(s) with linearized vector for five minutes at room temperature and ten minutes on ice. A typical cloning reaction will deliver a successful cloning rate of more than 90%.
- Fast—get transformation-ready DNA in as little as twenty minutes
- Easy—a phosphatase-free and ligation-free system that eliminates the need for specific restriction enzymes
- Efficient—typically achieve >90% positive clones
- Versatile—add any insert into any site in any vector using manual or automated workflows
“I have designed and made hundreds and hundreds of clones using the traditional and not so traditional methods since I started doing research. I must say the Cold Fusion kit makes it way too easy to design a vector or new construct—it’s a really good product.”
~Hidevaldo B. Machado, PhD., UCLA